6F0J
GLIC mutant E26A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 190 |
Detector technology | PIXEL |
Collection date | 2015-05-06 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 181.010, 133.110, 159.850 |
Unit cell angles | 90.00, 102.14, 90.00 |
Refinement procedure
Resolution | 25.000 - 3.150 |
R-factor | 0.2084 |
Rwork | 0.208 |
R-free | 0.22210 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.020 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.690 | 3.150 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 64088 | |
<I/σ(I)> | 10.2 | |
Completeness [%] | 99.8 | |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 292 | 12% PEG4K, 15% glycerol, 100 mM Acetate de Sodium pH4, 400 mM Sodium isothiocyanate, 3% DMSO |