6F04

N-acetylglucosamine-2-epimerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918400
Spacegroup name	P 42 21 2
Unit cell lengths	142.423, 142.423, 52.046
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.884 - 1.699
R-factor	0.1796
Rwork	0.179
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fp3
RMSD bond length	0.007
RMSD bond angle	1.061
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.800
High resolution limit [Å]	1.699	1.699
Rmerge	0.037	1.498
Number of reflections	59456
<I/σ(I)>	15.58
Completeness [%]	99.9
Redundancy	12
CC(1/2)	0.999	0.643

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	Sodium acetate 0,1 pH 5.0 PEG 3350 10% Dextran 3%