6EZV
The cytotoxin MakA from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-04 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.96770 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.575, 36.527, 73.551 |
| Unit cell angles | 90.00, 91.25, 90.00 |
Refinement procedure
| Resolution | 47.999 - 1.900 |
| R-factor | 0.1981 |
| Rwork | 0.195 |
| R-free | 0.24890 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.024 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.999 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.092 | 0.907 |
| Rpim | 0.047 | 0.460 |
| Number of reflections | 25893 | 1666 |
| <I/σ(I)> | 12.5 | 2.5 |
| Completeness [%] | 94.5 | 95.9 |
| Redundancy | 7.4 | 7.6 |
| CC(1/2) | 0.987 | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M sodium acetate, 24% (w/v) PEG 8000, 0.1 M sodium cacodylate pH 6.5. |
