6EZL
Crystal structure of aspartate aminotransferase from Trypanosoma cruzi at 2.07 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9801 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.495, 59.086, 139.015 |
| Unit cell angles | 90.00, 112.64, 90.00 |
Refinement procedure
| Resolution | 45.376 - 2.070 |
| R-factor | 0.1814 |
| Rwork | 0.180 |
| R-free | 0.22930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4h51 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.380 | 2.130 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.110 | 0.864 |
| Number of reflections | 54095 | |
| <I/σ(I)> | 7.3 | 1.6 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 0% PEG 6000 5% Glycerol 50mM Sodium Acetate pH 4.6 |
