6EZ7
Pes4 RRM3 Structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97623 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 82.826, 82.826, 31.985 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.212 - 1.900 |
| R-factor | 0.1962 |
| Rwork | 0.195 |
| R-free | 0.22440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d77 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.717 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.212 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.245 | |
| Rmeas | 0.255 | |
| Number of reflections | 10165 | |
| <I/σ(I)> | 6.63 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 294 | 200 mM Ammonium Sulfate, 30% PEG 8000, 1M MES/NaOH pH 7.4 (additive) |
