6EYY
Anti-CRISPR AcrIIa6 cubic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 175.270, 175.270, 175.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.840 - 2.500 |
| R-factor | 0.2323 |
| Rwork | 0.232 |
| R-free | 0.24520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmeas | 0.189 | 1.000 |
| Number of reflections | 32420 | 32420 |
| <I/σ(I)> | 13.3 | 2.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 26 | 26 |
| CC(1/2) | 0.995 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Magnesium chloride, 0.1M tris pH6.5-7.5, 5-15%(w/v) PEG 8000. |
