6EYW

Structure of Yeast Myosin 5 Cargo Binding Domain in Trigonal Space Group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9801
Spacegroup name	P 32 2 1
Unit cell lengths	63.830, 63.830, 227.691
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.726 - 2.880
R-factor	0.2244
Rwork	0.219
R-free	0.26890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f6h
RMSD bond length	0.008
RMSD bond angle	1.254
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.700	3.040
High resolution limit [Å]	2.880	2.880
Rmerge	0.108	0.905
Rmeas	0.119	0.992
Number of reflections	12921
<I/σ(I)>	14	2.3
Completeness [%]	99.9	100
Redundancy	5.6	6
CC(1/2)	0.998	0.638

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	20% PEG3000 0.1M Tris-Cl, pH 8