6EYH
Structure of a OpuBC mutant with bound Glycine betaine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-17 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.770, 65.890, 63.580 |
| Unit cell angles | 90.00, 92.14, 90.00 |
Refinement procedure
| Resolution | 63.500 - 1.600 |
| R-factor | 0.1713 |
| Rwork | 0.169 |
| R-free | 0.20983 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3r6u |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.050 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.500 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 32423 | 3238 |
| <I/σ(I)> | 12.4 | 4.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 5 | 273 | 150 to 225 mM potassium acetate and 20% to 27.5% polyethylene glycol 3350 |
