6EY5

C-terminal part (residues 224-515) of PorM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-04
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97888,0.979109,0.979336,0.976256
Spacegroup name	P 43 21 2
Unit cell lengths	77.004, 77.004, 228.557
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.420 - 2.850
R-factor	0.219
Rwork	0.217
R-free	0.24900
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.230
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	autoSHARP
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.000
High resolution limit [Å]	2.850	2.850
Rmeas	0.081	0.815
Rpim		0.276
Number of reflections	16929
<I/σ(I)>	14.8	2.2
Completeness [%]	99.9	100
Redundancy	8	8
CC(1/2)		0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.02 M sodium acetate pH 4.8-5.8 0.2 M zinc acetate 15-25%(w/v) PEG 3350