6EY4

Periplasmic domain (residues 36-513) of GldM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97908 , 0.9677
Spacegroup name	P 64 2 2
Unit cell lengths	71.377, 71.377, 426.941
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.420 - 2.000
R-factor	0.224
Rwork	0.222
R-free	0.25900
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.160
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	autoSHARP
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.660	2.110
High resolution limit [Å]	2.000	2.000
Rmeas	0.125	2.660
Rpim		0.694
Number of reflections	45389	6440
<I/σ(I)>	10.1	1.2
Completeness [%]	100.0	100
Redundancy	13.6	14.4
CC(1/2)		0.667

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Hepes pH7.0-8.0 26-46%(w/v) PEG 600