6EY0
N-terminal part (residues 30-212) of PorM with the llama nanobody nb01
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.283, 99.924, 80.291 |
| Unit cell angles | 90.00, 93.83, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.400 |
| R-factor | 0.214 |
| Rwork | 0.213 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lz0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.470 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmeas | 0.078 | 2.033 |
| Rpim | 0.774 | |
| Number of reflections | 49059 | 7020 |
| <I/σ(I)> | 11.6 | 1 |
| Completeness [%] | 98.3 | 96.7 |
| Redundancy | 6.9 | 6.8 |
| CC(1/2) | 0.755 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M bis-tris pH 7.0, 25%(w/v) PEG 3350 |
