6EXU

Crystal structure of the DNA binding domain of fission yeast Sap1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-21
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	35.582, 40.831, 70.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.390 - 1.409
R-factor	0.1703
Rwork	0.169
R-free	0.19820
RMSD bond length	0.008
RMSD bond angle	0.973
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.390	1.459
High resolution limit [Å]	1.400	1.409
Rmerge	0.021	0.189
Number of reflections	20477
<I/σ(I)>	24.76	4.62
Completeness [%]	99.5	96.52
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M SPG buffer (Succinic Acid, Phosphate, Glycine) pH 7.0, 25% PEG 1500