6EXT
Crystal structure of the DNA binding domain of fission yeast Sap1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-08 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.422, 40.773, 71.228 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.610 - 1.500 |
| R-factor | 0.1688 |
| Rwork | 0.169 |
| R-free | 0.21220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6exu |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.084 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.610 | 1.554 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.023 | 0.123 |
| Number of reflections | 17123 | |
| <I/σ(I)> | 5.64 | 6.21 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 5% w/v PEG 1500 and PCTP buffer pH 7.0 (propionic acid, cacodylate and bis-tris propane) |
