6EWW

Structure of 14-3-3 zeta in complex with CaMKK2 14-3-3 binding motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	56.947, 60.309, 262.185
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.712 - 2.679
R-factor	0.2091
Rwork	0.208
R-free	0.23450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fj3
RMSD bond length	0.009
RMSD bond angle	0.969
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.890	2.840
High resolution limit [Å]	2.679	2.679
Rmeas	0.080	0.300
Number of reflections	26274	4126
<I/σ(I)>	17.37	5.21
Completeness [%]	99.7	98.9
Redundancy	6.42	6.23
CC(1/2)	0.998	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291.15	PEG 2000, TRIS-HCl, HEPES, magnesium chloride, sodium fluoride, TCEP