6EWR
Putative sugar aminotransferase Spr1654 from Streptococcus pneumoniae, PMP-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.899, 99.562, 108.095 |
| Unit cell angles | 90.00, 97.53, 90.00 |
Refinement procedure
| Resolution | 29.800 - 2.400 |
| R-factor | 0.245 |
| Rwork | 0.242 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lc3 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.531 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.800 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.151 | 0.616 |
| Number of reflections | 64471 | 6344 |
| <I/σ(I)> | 7.4 | 2 |
| Completeness [%] | 99.0 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 0.2 M Sodium acetate trihydrate 0.1 M Sodium cacodylate trihydrate pH 6.5 30% w/v Polyethylene glycol 8 000 |
