6EWO

Crystal structure of non-phosphorylated form of RTF PHOSPHOPEPTIDE BOUND TO HLA-A2 in complex with LILRB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-25
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5417
Spacegroup name	P 32 2 1
Unit cell lengths	116.300, 116.300, 192.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.700 - 2.300
R-factor	0.2344
Rwork	0.232
R-free	0.27640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p7q
RMSD bond length	0.008
RMSD bond angle	1.291
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.700	19.700	2.400
High resolution limit [Å]	2.300	10.000	2.300
Rmerge	0.100	0.020	0.734
Rmeas	0.105	0.021	0.807
Total number of observations	758810
Number of reflections	66969	796	7529
<I/σ(I)>	23.61	88.88	2.82
Completeness [%]	99.1	84.9	94
Redundancy	11.331	10.866	5.538

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% PEG 3350, 0.2M Potassium sodium tartrate