6EWL

Danio rerio CEP120 first C2 domain (C2A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	31.459, 53.440, 74.659
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.603 - 1.400
R-factor	0.1647
Rwork	0.163
R-free	0.20210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	C2 domain homology model
RMSD bond length	0.012
RMSD bond angle	1.222
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.460	1.480
High resolution limit [Å]	1.400	1.400
Rmerge	0.049
Rmeas	0.054
Rpim	0.022	0.435
Number of reflections	25380
<I/σ(I)>	14.1	1.7
Completeness [%]	99.3	98.1
Redundancy	5.9	6.1
CC(1/2)	0.999	0.533

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	100 mM HEPES, pH 7.5 200 mM MgCl2 30 % (v/v) PEG-400