6EWJ
Putative sugar aminotransferase Spr1654 from Streptococcus pneumoniae, apo-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-12 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.330, 99.982, 110.422 |
| Unit cell angles | 90.00, 97.66, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.238 |
| Rwork | 0.236 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lc3 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.572 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.063 | 0.444 |
| Number of reflections | 130980 | 13145 |
| <I/σ(I)> | 10.9 | 2.3 |
| Completeness [%] | 97.0 | |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 0.2 M Sodium acetate trihydrate 0.1 M Sodium cacodylate trihydrate pH 6.5 30% w/v Polyethylene glycol 8 000 |
