6EWI

Oreochromis niloticus CEP120 second C2 domain (C2B) A200P + G307S mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-27
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.87260
Spacegroup name	P 21 21 21
Unit cell lengths	40.726, 95.449, 99.168
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.004 - 2.100
R-factor	0.2337
Rwork	0.232
R-free	0.27390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ewh
RMSD bond length	0.003
RMSD bond angle	0.606
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.000	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.275
Rmeas	0.295
Rpim	0.103	0.532
Number of reflections	23375	3359
<I/σ(I)>	5.7	1.6
Completeness [%]	100.0	100
Redundancy	8	8
CC(1/2)	0.992	0.687

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	292	100 mM Tris, pH 8.0 30 % (w/v) PEG-6000