6EWG

Oreochromis niloticus CEP120 second C2 domain (C2B)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	36.389, 67.488, 89.119
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.186 - 1.600
R-factor	0.1863
Rwork	0.185
R-free	0.20280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ewh
RMSD bond length	0.005
RMSD bond angle	0.811
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.120	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.062
Rmeas	0.065
Rpim	0.018	0.536
Number of reflections	29804	1461
<I/σ(I)>	15.5	1.6
Completeness [%]	100.0	100
Redundancy	12.7	13.2
CC(1/2)	0.999	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	0.1 Na-MES pH ~6.5 30% PEG 400