6EWA
Crystal structure of HLA-A2 in complex with LILRB1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93927 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 116.150, 116.150, 192.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.590 - 2.390 |
| R-factor | 0.2306 |
| Rwork | 0.228 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p7q |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.264 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.21) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.679 | 48.679 | 2.520 |
| High resolution limit [Å] | 2.390 | 7.560 | 2.390 |
| Rmerge | 0.122 | 0.031 | 0.537 |
| Rmeas | 0.130 | 0.033 | 0.567 |
| Rpim | 0.042 | 0.011 | 0.182 |
| Number of reflections | 60013 | 2081 | 8505 |
| <I/σ(I)> | 17.1 | 17.4 | 1.4 |
| Completeness [%] | 99.6 | 99.6 | 97.7 |
| Redundancy | 9.6 | 8.9 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 17.5% PEG 3350, 0.2M Ammonium acetate, 0.1M Tris HCL |
