6EVJ
Crystal structure of bat influenza A/H17N10 polymerase with viral RNA promoter and capped RNA primer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.460, 286.170, 171.930 |
| Unit cell angles | 90.00, 93.27, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.900 |
| R-factor | 0.20857 |
| Rwork | 0.207 |
| R-free | 0.24530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wsb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.963 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.000 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmeas | 0.137 | 1.440 |
| Number of reflections | 81025 | |
| <I/σ(I)> | 8.28 | 0.93 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 3.87 | 3.87 |
| CC(1/2) | 0.998 | 0.539 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 281 | 0.1 M bicine pH 9.0, 17% MPD |
