6EUS
Crystal structure of the outer membrane channel DcaP of Acinetobacter baumannii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 107.847, 108.382, 216.202 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 108.100 - 2.200 |
| R-factor | 0.20065 |
| Rwork | 0.200 |
| R-free | 0.23362 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.041 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.440 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.100 | 0.739 |
| Number of reflections | 125363 | 6276 |
| <I/σ(I)> | 6 | 1.4 |
| Completeness [%] | 97.5 | 99.1 |
| Redundancy | 2.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 8.5% MPD, 8.5% PEG 1000, 8.5% PEG 3350, 0.02 M sodium formate, 0.02 M sodium citrate, 0.02 M sodium oxamate, 0.02 M ammonium acetate, 0.02 M sodium potassium tartrate, 0.05 M MES, 0.05 M imidazole pH 6.5 |
