6EUI
The GH43, Beta 1,3 Galactosidase, BT3683 with galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97 |
| Spacegroup name | H 3 |
| Unit cell lengths | 136.380, 136.380, 53.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 68.190 - 1.760 |
| R-factor | 0.17021 |
| Rwork | 0.168 |
| R-free | 0.21231 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BT0265 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.508 |
| Data reduction software | HKL-3000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 68.190 |
| High resolution limit [Å] | 1.760 |
| Number of reflections | 36549 |
| <I/σ(I)> | 11.5 |
| Completeness [%] | 99.5 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 3350 0.2 M Ammonium Formate |
