6EUF

The GH43, Beta 1,3 Galactosidase, BT0265

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.93
Spacegroup name	P 21 21 21
Unit cell lengths	106.000, 119.700, 182.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.040 - 2.200
R-factor	0.21135
Rwork	0.208
R-free	0.27086
RMSD bond length	0.017
RMSD bond angle	1.773
Data reduction software	HKL-3000
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.400
High resolution limit [Å]	2.200
Number of reflections	117960
<I/σ(I)>	13.3
Completeness [%]	100.0
Redundancy	13.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20 % PEG 3350 0.2 M Sodium Thiocyanate