6EUB

The fibrinogen-like domain of human Angptl4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-31
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.99987
Spacegroup name	C 2 2 21
Unit cell lengths	133.020, 133.770, 39.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.162 - 2.300
R-factor	0.2035
Rwork	0.201
R-free	0.25350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z3s
RMSD bond length	0.010
RMSD bond angle	1.054
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.162	47.162	2.360
High resolution limit [Å]	2.300	10.270	2.300
Rmerge	0.145	0.059	1.139
Rmeas	0.158	0.065	1.245
Number of reflections	16070	213	1060
<I/σ(I)>	8.36	23.17	1.31
Completeness [%]	98.6	96.8	89.9
Redundancy	6.396	5.61	5.858
CC(1/2)	0.995	0.998	0.633

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	295	0.1 M Bis-Tris Propane pH 7.5, 0.2 M Sodium malonate, 20 % (w/v) PEG 3350