6EUA

The fibrinogen-like domain of human Angptl3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.966
Spacegroup name	P 21 21 21
Unit cell lengths	60.050, 63.650, 169.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.990 - 2.095
R-factor	0.1968
Rwork	0.194
R-free	0.25370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z3s
RMSD bond length	0.007
RMSD bond angle	0.774
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.990	48.990	2.150
High resolution limit [Å]	2.100	9.370	2.100
Rmerge	0.129	0.049	1.284
Rmeas	0.150	0.058	1.488
Total number of observations	148692
Number of reflections	38790	497	2742
<I/σ(I)>	7.16	23.32	1
Completeness [%]	99.3	96.5	96.1
Redundancy	3.833	3.497	3.786
CC(1/2)	0.994	0.995	0.334

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	295	0.2 M Magnesium chloride hexahydrate, 0.1 M BIS-Tris pH 5.5, 25 % (w/v) PEG 3350