6EU8
Crystal structure of Tannerella forsythia Apo HmuY analog (TFO)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92818 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 68.050, 68.050, 90.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.340 - 1.470 |
| R-factor | 0.18631 |
| Rwork | 0.185 |
| R-free | 0.21908 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uu2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.502 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.340 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.033 | 2.100 |
| Rpim | 0.011 | 0.760 |
| Number of reflections | 41705 | 2033 |
| <I/σ(I)> | 30.2 | 1.1 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 9.8 | 9 |
| CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | Sodium malonate, Tris pH 7.5 |
