6ETZ

Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 31 2 1
Unit cell lengths	137.784, 137.784, 127.208
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.730 - 1.800
R-factor	0.1618
Rwork	0.161
R-free	0.19750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yq2
RMSD bond length	0.019
RMSD bond angle	1.874
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.730	46.730	1.910
High resolution limit [Å]	1.800	5.360	1.800
Rmerge	0.107	0.037	1.288
Rmeas	0.113	0.039	1.361
Total number of observations	1287351
Number of reflections	128996	5133	20271
<I/σ(I)>	14.46	48.57	1.51
Completeness [%]	99.5	99.7	97.5
Redundancy	9.98	9.423	9.59
CC(1/2)	0.999	0.999	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	35% Tacsimate pH 8.0