6ETP
Atomic resolution structure of RNase A (data collection 6)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-09-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.7749 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 29.125, 37.971, 52.541 |
Unit cell angles | 90.00, 105.83, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.020 |
R-factor | 0.1456 |
Rwork | 0.140 |
R-free | 0.17800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kf3 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | SHELXL |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.060 |
High resolution limit [Å] | 1.020 | 1.020 |
Rmerge | 0.056 | 0.431 |
Number of reflections | 51404 | |
<I/σ(I)> | 8.65 | 2.47 |
Completeness [%] | 91.9 | 89.6 |
Redundancy | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 293 | Free-interface diffusion of a 10 uL of 30 mg ml-1 protein solution, 20 mM sodium citrate at pH 5.3 agaist 10 ul isopropanol 99.9% isopropanol. |