6ERU
Crystal Structure of the Protein-Kinase A catalytic subunit from Criteculus Griseus in complex with compound RKp120
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.999, 72.966, 109.004 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.878 - 2.150 |
R-factor | 0.2072 |
Rwork | 0.205 |
R-free | 0.24480 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.800 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.878 | 2.280 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 26202 | 4116 |
<I/σ(I)> | 21.13 | 3.02 |
Completeness [%] | 99.2 | 98.4 |
Redundancy | 4.7 | 4.77 |
CC(1/2) | 0.999 | 0.854 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 277.15 | 100 mM Mes-Bis-Tris, 75 mM lithium chloride, 1 mM DTT, 0.1 mM sodium EDTA, 0.25 mM Mega 8, 0.7 mM peptidic ligand, 19% v/v methanol/water in reservoir |