6ERO
Structure of human TFB2M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.950, 165.650, 44.730 |
| Unit cell angles | 90.00, 97.97, 90.00 |
Refinement procedure
| Resolution | 43.525 - 1.750 |
| R-factor | 0.1931 |
| Rwork | 0.192 |
| R-free | 0.21380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Truncated model of S.cerevisiae Mtf1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.680 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.530 | 1.790 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmeas | 0.048 | 2.874 |
| Number of reflections | 62109 | 4544 |
| <I/σ(I)> | 20.17 | 0.75 |
| Completeness [%] | 97.8 | 97.5 |
| Redundancy | 7.1 | 7 |
| CC(1/2) | 1.000 | 0.377 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | NaCl, PEG3350, HEPES |
