6ERD
Crystal structure of a putative acetyltransferase from Bacillus cereus species.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.520, 84.170, 72.080 |
| Unit cell angles | 90.00, 98.49, 90.00 |
Refinement procedure
| Resolution | 44.970 - 2.000 |
| R-factor | 0.1657 |
| Rwork | 0.164 |
| R-free | 0.20103 |
| Structure solution method | MAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.099 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.970 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.090 | 0.716 |
| Rmeas | 0.104 | 0.834 |
| Rpim | 0.053 | 0.425 |
| Number of reflections | 60997 | |
| <I/σ(I)> | 15.5 | 2.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 30%PEG 6000 100mM Hepes pH7.0 1M LiCl 10 mM BME |
