6ERB

Enterococcus faecalis FIC protein (H111A) in complex with sulfate.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980112
Spacegroup name	I 2 2 2
Unit cell lengths	121.931, 131.161, 136.711
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.046 - 2.200
R-factor	0.215
Rwork	0.213
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g03
RMSD bond length	0.008
RMSD bond angle	1.012
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.320	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.081	1.321
Rpim	0.032	0.526
Number of reflections	55829	4558
<I/σ(I)>	18.5	2.1
Completeness [%]	100.0	100
Redundancy	13.7	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	50mM Tris pH 8.0, 150mM NaCl, 2mM ATPgS, 2mM MgCl2, 0.2M Lithium Sulfate, 0.1M Tris pH 8.5, 20% (w/v) PEG 4000