6ER1

Crystal structure of BTB-domain of CP190 from D.melanogaster at high resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1,0
Spacegroup name	P 32 2 1
Unit cell lengths	84.940, 84.940, 40.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	73.560 - 1.400
R-factor	0.1476
Rwork	0.146
R-free	0.18070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4u77
RMSD bond length	0.018
RMSD bond angle	1.908
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	73.560	73.560	1.420
High resolution limit [Å]	1.400	7.670	1.400
Rmerge	0.044	0.070	0.581
Rmeas	0.050	0.080	0.664
Rpim	0.024	0.039	0.318
Number of reflections	32673	238	1639
<I/σ(I)>	15.5
Completeness [%]	98.2	99.9	99.9
Redundancy	4.1	4.3	4.2
CC(1/2)	0.997	0.979	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		293	0.1M HEPES pH 7.5; 0.8M Ammonium phosphate monobasic; 0.8 Potassium phosphate monobasic