6EQ5

MTH1 in complex with fragment 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	60.804, 66.467, 36.242
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.864 - 1.801
R-factor	0.2155
Rwork	0.209
R-free	0.27050
RMSD bond length	0.006
RMSD bond angle	0.772
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.910
High resolution limit [Å]	1.800	1.800
Rmeas	0.073
Number of reflections	24073	4073
<I/σ(I)>	9.32	2.64
Completeness [%]	91.5	95.6
Redundancy	1.77	1.77
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	23 % PEG3350, 0.2 M LI2SO4, 0.1 M SODIUM ACETATE PH4.5