6EOA

Crystal Structure of HAL3 from Cryptococcus neoformans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-15
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9792
Spacegroup name	H 3 2
Unit cell lengths	88.194, 88.194, 165.774
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.168 - 2.180
R-factor	0.2158
Rwork	0.214
R-free	0.23910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e20
RMSD bond length	0.004
RMSD bond angle	0.883
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.170	2.290
High resolution limit [Å]	2.180	2.180
Rmerge	0.062
Rmeas	0.068
Rpim	0.030
Number of reflections	12855	1694
<I/σ(I)>	17.2
Completeness [%]	96.5	89.2
Redundancy	9.4	6.9
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291	0.8M Succinic acid, pH 7.0