6EO2
Conformational dynamism for DNA interaction in Salmonella typhimurium RcsB response regulator. S207C crossed
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-11 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.91587 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 92.952, 92.952, 120.721 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 80.500 - 2.600 |
| R-factor | 0.22059 |
| Rwork | 0.219 |
| R-free | 0.24587 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.018 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.600 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.061 | 1.241 |
| Number of reflections | 195331 | |
| <I/σ(I)> | 31.8 | 2.6 |
| Completeness [%] | 96.4 | 81 |
| Redundancy | 20.2 | 17.8 |
| CC(1/2) | 1.000 | 0.826 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 30 % polyacrylate 2100, 0.1 M sodium malonate and 0.1 M Hepes pH 7.0 |
