6ENZ
Crystal structure of mouse GADL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.430, 80.640, 128.510 |
| Unit cell angles | 90.00, 117.85, 90.00 |
Refinement procedure
| Resolution | 42.184 - 3.000 |
| R-factor | 0.2381 |
| Rwork | 0.235 |
| R-free | 0.28760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jis |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmeas | 0.328 | 3.535 |
| Number of reflections | 25036 | |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 99.4 | 99.5 |
| Redundancy | 6.4 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 80 mM Na-cacodylate, pH 6.0, 14% PEG 8000, 160 mM Ca-acetate, 15% glycerol |
