6ENE
OmpF orthologue from Enterobacter cloacae (OmpE35)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 137.548, 187.253, 122.957 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.835 - 2.300 |
| R-factor | 0.2081 |
| Rwork | 0.206 |
| R-free | 0.24680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zfg |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.405 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.810 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rpim | 0.030 | 0.560 |
| Number of reflections | 70576 | 4485 |
| <I/σ(I)> | 16 | 1.4 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.6 | 7.5 |
| CC(1/2) | 0.990 | 0.630 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.05M Citric Acid 0.1M Lithium sulfate 0.05M Sodium phosphate dibasic 19% PEG 1000 |
