6EN8
SaFadR in complex with dsDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2015-02-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.850, 178.310, 266.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.410 - 3.290 |
| R-factor | 0.2037 |
| Rwork | 0.202 |
| R-free | 0.23830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5MWR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.410 | 3.380 |
| High resolution limit [Å] | 3.290 | 3.290 |
| Rmeas | 0.095 | 0.746 |
| Number of reflections | 40698 | 2851 |
| <I/σ(I)> | 12.59 | 1.96 |
| Completeness [%] | 99.5 | 95.7 |
| Redundancy | 6.8 | 6.5 |
| CC(1/2) | 0.958 | 0.953 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Tris pH 8, 0.4M Lithium chloride, 15% PEG 6000 |
