6EN7

Crystal structure of the ribosome assembly factor Nsa1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	54.723, 81.100, 87.574
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.408 - 2.403
R-factor	0.1801
Rwork	0.178
R-free	0.21980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5sum
RMSD bond length	0.002
RMSD bond angle	0.516
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.500	2.489
High resolution limit [Å]	2.400	2.400
Rmerge	0.044	0.113
Number of reflections	15750	1543
<I/σ(I)>	34.78	14.02
Completeness [%]	99.9	99.23
Redundancy	7.1	5.6
CC(1/2)	0.999	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.1 M MES pH 6.5, 25% w/v PEG-1000