6EMF
Crystal structure of Rrp1 from Chaetomium thermophilum in space group C2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.720, 74.675, 68.795 |
| Unit cell angles | 90.00, 90.53, 90.00 |
Refinement procedure
| Resolution | 47.791 - 2.650 |
| R-factor | 0.2004 |
| Rwork | 0.199 |
| R-free | 0.23180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.495 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.791 |
| High resolution limit [Å] | 2.650 |
| Number of reflections | 19400 |
| <I/σ(I)> | 6.88 |
| Completeness [%] | 99.2 |
| Redundancy | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 200 mM Proline 100 mM HEPES pH 7.5 10% PEG3350 |
