6EM0
Crystal Structure of 2-hydroxybiphenyl 3-monooxygenase M321A from Pseudomonas azelaica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-02-16 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.56 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.570, 130.960, 79.080 |
| Unit cell angles | 90.00, 98.96, 90.00 |
Refinement procedure
| Resolution | 50.182 - 2.780 |
| R-factor | 0.2277 |
| Rwork | 0.226 |
| R-free | 0.25290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z2r |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.908 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.770 | 2.930 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.108 | 0.308 |
| Rmeas | 0.136 | 0.397 |
| Rpim | 0.082 | 0.246 |
| Number of reflections | 34717 | 4810 |
| <I/σ(I)> | 6 | 2.4 |
| Completeness [%] | 88.9 | 84.6 |
| Redundancy | 2.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M sodium citrate 25% PEG3350 |
