6ELV
Recombinantly expressed C-terminal domain of MdPPO1 (Csole-domain)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9724 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.130, 50.100, 50.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.581 - 1.050 |
| R-factor | 0.1482 |
| Rwork | 0.148 |
| R-free | 0.16070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | latent MdPPO1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.987 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.540 | 1.080 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.549 | |
| Rmeas | 0.016 | 0.248 |
| Rpim | 0.058 | 0.572 |
| Number of reflections | 51007 | 3333 |
| <I/σ(I)> | 20.85 | 2.4 |
| Completeness [%] | 94.1 | 54 |
| Redundancy | 11.3 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15 mg/ml, 100 mM TRIS-HCl pH 8.25, 13 % PEG8000 |
