6ELU

Structure of Serum Resistance Associated protein from T. b. rhodesiense

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	127.600, 103.550, 150.640
Unit cell angles	90.00, 114.58, 90.00

Refinement procedure

Resolution	48.431 - 2.300
R-factor	0.192
Rwork	0.191
R-free	0.22040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.002
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	48.431
High resolution limit [Å]	2.300
Number of reflections	310049
<I/σ(I)>	9.5
Completeness [%]	99.4
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20 mM sodium formate, 20 mM ammonium acetate, 20 mM sodium citrate tribasic dehydrate, 20 mM sodium potassium tartrate tetrahydrate, 20 mM sodium oxamate, 100 mM tris(base)/Bicine pH 8.5, 20% (v/v) PEG 500 MME, 10 % (w/v) PEG 20,000