6ELT
C-terminal domain of MdPPO1 upon self-cleavage (Ccleaved-domain)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-25 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.230, 50.140, 50.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.658 - 1.350 |
| R-factor | 0.1724 |
| Rwork | 0.171 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | latent MdPPO1 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.801 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.660 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.832 | |
| Rmeas | 0.042 | 0.422 |
| Rpim | 0.093 | 0.937 |
| Number of reflections | 25835 | 2542 |
| <I/σ(I)> | 10.41 | 1.7 |
| Completeness [%] | 99.4 | 99.6 |
| Redundancy | 4.5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15 mg/ml enzyme, 100 mM TRIS-HCl pH 8.25, 13 % PEG8000 |
