6ELJ
FAB Fragment. AbVance: Increasing our knowledge of antibody structural space to enable faster and better decision making in antibody drug discovery
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.578, 67.301, 76.615 |
| Unit cell angles | 116.76, 89.17, 105.07 |
Refinement procedure
| Resolution | 44.240 - 1.900 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | in house Fab structure |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.240 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.010 | |
| Number of reflections | 67823 | |
| <I/σ(I)> | 7.55 | |
| Completeness [%] | 97.6 | 96.2 |
| Redundancy | 3.42 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Na cacodylat 6.5 pH 25 %w/v PEG 4K |
