6ELD

Crystal structure of TIA-1 RRM1 in complex with U1C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.92
Spacegroup name	P 64
Unit cell lengths	71.110, 71.110, 60.680
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.223 - 2.485
R-factor	0.1992
Rwork	0.197
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5o2v
RMSD bond length	0.001
RMSD bond angle	0.339
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.223	43.223	2.550
High resolution limit [Å]	2.485	11.110	2.490
Rmerge	0.061	0.024	0.763
Rmeas	0.066	0.026	0.836
Number of reflections	6053	69	401
<I/σ(I)>	16.85	49.06	1.81
Completeness [%]	96.9	88.5	86.6
Redundancy	6.16	6.203	5.514
CC(1/2)	0.999	1.000	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1 M HEPES, 10% PEG6000 and 10% MPD