6EKR

Crystal structure of Type IIP restriction endonuclease Kpn2I

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98019,0.98029,0.97789
Spacegroup name	I 21 3
Unit cell lengths	134.794, 134.794, 134.794
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.880
R-factor	0.21498
Rwork	0.209
R-free	0.26398
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.140
Data reduction software	XDS
Data scaling software	SCALA (CCP4 6.2: Scala version 3.3.20 : 23/06/11)
Phasing software	Auto-Rickshaw
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.310	3.040
High resolution limit [Å]	2.880	2.880
Rmerge	0.116	0.537
Rmeas	0.134	0.555
Rpim	0.021	0.093
Number of reflections	9398	1347
<I/σ(I)>	28.3	7.3
Completeness [%]	100.0	100
Redundancy	39.8	34.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	Crystallization buffer: 0.1M NaHepes pH7.5, 1.4M ammonium sulfate and glycerol 10%. Complex of Kpn2I with DNA was used at concentration 2-7.5mg/ml